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Media from the apical compartment was removed, and the cells were carefully washed four times with PBS, and the lysed with 5% IGEPAL (Sigma-Aldrich) in water

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This process is mediated by a complex reflex involving a swallowing centre in the dorsal motor nucleus of the vagus in the brainstem

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These processes converge on circadian rhythm dysfunction, which is further reinforced by oxidative stress, increased reactive oxygen species (ROS) production, and alterations in circadian clock gene expression